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| 论文摘要： | In birds and mammals, the gonadotrophin-inhibitory hormone (GnIH) effectively inhibits the expression of gonadotrophin (GtH). In teleosts, the effects of GnIH are still unclear and under much debate. The aim of this study is to evaluate the functions of GnlH/receptors of gonadotrophin-inhibitory hormone (GnIHRs) system during reproduction in the common carp, Cyprinus carpio L We cloned the full cDNA sequences of GnIH (GnIHRs. Real-time PCR results showed that GnIH/GnIHRs were distributed extensively across the whole hypothalamus-pituitary-gonad (HPG) axis. We also examined the changes of GnIH/GnIHRs in the HPG axis during reproduction. GnIH mRNA expression was decreased to the minimum value in Apr, the spawning month, and increased immediately after the completion of reproduction. Expression pattern of GnIH during reproduction was the opposite to those of Gonadotrophin release hormone 3 (GnRH3) and luteinizing hormone (LH). Expression patterns of GnIHRs were similar to that of GnIH in the hypothalamus. In the pituitary, GnIHR2/3 peaked in March before spawning. In the ovaries, the GnIHR1 decreased to the minimum value in April, but GnIHR2/3 increased. By injection and incubation with synthesized GnIH-III peptide, we confirmed the negative influence of GnIH on mRNAs of the follicle-stimulating hormone-beta and LH-beta subunits in the common carp. These results show that the GnIH/GnIHRs system is involved in the negative regulation of reproduction in HPG axis of the common carp. (C) 2016 Elsevier Inc. All rights reserved. |
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