| 论文标题： | |
|---|---|
| 作者： | |
| 出版刊物： | |
| 出版日期： | |
| 出版年份： | |
| 卷/期： | |
| DOI： | |
| 论文摘要： | Grass carp is an important commercial fish in China that is plagued by various diseases, especially the hemorrhagic disease induced by grass carp reovirus (GCRV). Autophagy, a highly conserved biological process among eukaryotes, is pivotal in maintaining cellular homeostasis and managing various stressors, including viral infections. Uncoordinated (Unc) 51-like kinase 2 (ULK2) is considered an initiator of the autophagic process. In this study, we successfully cloned and isolated the ULK2 gene from grass carp. We observed that its expression levels were markedly altered following exposure to GCRV or pathogen-associated molecular patterns (PAMPs). Overexpression of CiULK2 in grass carp ovary cells (GCO) promoted GCRV replication. Conversely, CiULK2 knockdown resulted in inhibited viral loads compared to the control group. Moreover, we also reveal that 2aminoadipic acid (2-AAA), a representative autophagy related metabolite, can inhibit autophagy and viral replication. Notably, these roles of CiULK2 in autophagy and GCRV replication were reversed upon treatment with the 2-AAA. Collectively, our findings demonstrate the 2-AAA regulates CiULK2 to inhibit GCRV replication. |
以下内容与「星空体育彩票怎么用」同属公开资讯，可按栏目接着查阅相关条目。
本站按公开材料组织页面。需要原文时请核对应栏目发布页。
若从搜索引擎进入，可先确认当前栏目名称，再按需打开相关阅读。
