| 论文标题： | |
|---|---|
| 作者： | |
| 出版刊物： | |
| 出版日期： | |
| 出版年份： | |
| 卷/期： | |
| DOI： | |
| 论文摘要： | Ubiquitination is a widely occurring reversible post-translational modification process in cells. RANBP2-type and C3HC4-type zinc finger-containing 1 (RBCK1) is a kind of E3 ubiquitin ligase. It regulates various biological functions, including protein degradation and kinase signaling pathways in mammals. However, the role of RBCK1 in the virus-induced innate immune response remains largely unknown, particularly in teleost. In this study, we demonstrated that the expression of Carassius auratus var Pengze RBCK1 (CapRBCK1) was induced by grass carp reovirus (GCRV) and poly (I:C) stimulation in tissues and cells. CapRBCK1 suppressed the replication of GCRV in cells. Overexpression of CapRBCK1 activated IFN1 response, whereas knockdown of CapRBCK1 inhibited this response. CapRBCK1 is primarily localized in the cytoplasm, where it directly interacted with IRF7 and thereby increased the phosphorylation and dimerization of IRF7 in GCRV-infected cells. Furthermore, CapRBCK1 catalyzed K63-linked ubiquitination of IRF7 at lysine 106. Finally, the mutant of CapRBCK1 RING domain exhibited a significant impairment to IRF7 ubiquitination. So, the RING domain is essential for CapRBCK1 enhancing IFN1 activation. This study provides, to our knowledge, some novel insights into the role of RBCK1 in innate immune response. |
以下内容与「星空体育彩票官网正规渠道」同属公开资讯，可按栏目接着查阅相关条目。
本站按公开材料组织页面。需要原文时请核对应栏目发布页。
若从搜索引擎进入，可先确认当前栏目名称，再按需打开相关阅读。
