| 论文标题： | |
|---|---|
| 作者： | |
| 出版刊物： | |
| 出版日期： | |
| 出版年份： | |
| 卷/期： | |
| DOI： | |
| 论文摘要： | Grass carp reovirus (GCRV), a double-stranded RNA virus, has been identified as the causative pathogen of grass carp hemorrhagic disease (GCHD), which is one of the most severe diseases in freshwater aquaculture in China. Previously, we reported that the von Hippel-Lindau protein (pVHL) suppresses antiviral innate immunity during RNA virus infection by targeting the mitochondrial antiviral signaling (MAVS) protein for degradation. However, the role of pVHL in the innate immunity against GCRV is still not clear. In the present study, the VHL gene of grass carp was cloned, and the VHL gene was found to be conserved throughout the species. Using zebrafish as a model, we showed that disruption of vhl facilitates antiviral gene expression during GCRV infection. After GCRV infection or poly I:C stimulation, the mRNA level of Ci-VHL was significantly increased. Furthermore, Ci-pVHL interacts with Ci-MAVS, promotes degradation of Ci-MAVS, and inhibits Ci-MAVS-mediated interferon (IFN) activation. In addition, we screened 249 grass carp samples and identified three different types of single nucleotide polymorphisms (SNPs) within the Ci-VHL gene. The effect of each SNP on antiviral gene expression and virus replication following GCRV infection was also investigated. To conclude, the findings presented here may provide valuable insights regarding the function of pVHL in innate immunity against GCRV. Furthermore, VHL may serve as a promising target for the breeding of anti-GCRV grass carp strains. |
以下内容与「星空体育彩票最新版下载」同属公开资讯，可按栏目接着查阅相关条目。
本站按公开材料组织页面。需要原文时请核对应栏目发布页。
若从搜索引擎进入，可先确认当前栏目名称，再按需打开相关阅读。
