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| 论文摘要： | The common carp (Cyprinus carpio) is listed as one of the 100 most threatening alien invasive species in the world, and the Trojan Y chromosome (TYC) strategy has significant potential for the eradication of this invasive species. The viability and fertility of released M-YY individuals are important factors that significantly affect the effectiveness of the TYC strategy, and have not yet been well characterized. In this study, we established a full-sib family consisting of XX, XY, and YY fish by crossing XY pseudo-females with XY males, and then conducted a systematic comparative study of M-XY and M-YY fish. Genotype statistics and growth tracking results showed that M-YY fish had viability and growth characteristics similar to those of M-XY fish. Histological and fecundity analyses revealed that sexually mature M-YY fish had a testicular structure, sperm volume and concentration, and sperm structure under an electron microscope similar to those of their M-XY counterparts, thereby indicating that adult M-YY had a fecundity equivalent to that of M-XY common carp. Temporal histological sections of gonadal tissues showed that prepubertal M-YY fish had significantly better spermatogenic capability than M-XY fish during the critical window of meiosis, and RT-qPCR analysis revealed that the expression levels of genes related to male differentiation, meiosis, and steroid hormone synthesis were significantly higher in M-YY fish. Consistent with this, liquid chromatography-mass spectrometry revealed that the M-YY common carp had higher serum testosterone levels during meiosis. Immunofluorescence analysis also showed that the expression level of the meiosis-related gene SCP3 was significantly higher in the M-YY common carp. This suggests that prepubertal YY fish have more active spermatogenesis and reach sexual maturity faster than XY fish. In summary, this study found that M-YY fish had comparable survivability and fecundity to M-XY fish and that prepubertal YY common carp matured faster and showed more active spermatogenesis, which provides a theoretical basis for the application of the TYC strategy to prevent and control biological invasions of common carp. |
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