| 论文标题： | |
|---|---|
| 作者： | |
| 出版刊物： | |
| 出版日期： | |
| 出版年份： | |
| 卷/期： | |
| DOI： | |
| 论文摘要： | Peptidoglycan recognition proteins (PGRPs) are evolutionarily conserved pattern recognition receptors critical for antibacterial immunity across vertebrates and invertebrates. In this study, two long-type (CaPGRP-L1, -L2) and one short-type (CaPGRP-S) PGRP genes were identified in snakehead (Channa argus), an economically important aquaculture species. Sequence analysis revealed that all three PGRPs contain a conserved Zn2*-binding site (composed of two His, one Tyr, and one Cys), essential for amidase activity. Tissue distribution profiling showed distinct expression patterns, with high transcript levels detected in liver, intestine or gill. Functional assays demonstrated that recombinant CaPGRP-L1, -L2, and -S bound peptidoglycan (PGN), displayed Zn2*dependent amidase activity, and inhibited Aeromonas hydrophila infection. Furthermore, three PGRPs activated NF-kappa B promoter reporter plasmid in a dose-dependent manner, while NF-kappa B subunits c-Rel and p65 upregulated the transcriptional activity of CaPGRP-L1, -L2, and -S luciferase promoters, suggesting that snakehead PGRPs are involved in NF-kappa B signaling. Collectively, this study has explored the function of snakehead PGRPs, and deepens our understanding on innate immune mechanisms in fish, which should provide a theoretical foundation for the application of snakehead PGRPs in preventing and controlling diseases in aquaculture. |
以下内容与「星空体育彩票官网安全访问」同属公开资讯，可按栏目接着查阅相关条目。
本站按公开材料组织页面。需要原文时请核对应栏目发布页。
若从搜索引擎进入，可先确认当前栏目名称，再按需打开相关阅读。
